FlowVeda Science
KSM-66 Ashwagandha
Clinical Research and Study Summaries
Below you will find summaries of 25 peer-reviewed clinical studies examining ashwagandha (Withania somnifera), including studies using the KSM-66® extract. Each summary includes the study title, authors, journal citation, key findings, and a representative excerpt. Where applicable, studies using the specific KSM-66® extract are distinguished from general ashwagandha research. FlowVeda® publishes this research for full transparency.
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
Research Domains
25 clinical studies organized across 5 research domains. Select a domain to explore the studies.
3 Studies
Cognitive Function
Memory, executive function, attention, and information processing speed
4 Studies
Stress Response
Cortisol reduction, stress resilience, quality of life, and weight management under stress
5 Studies
Neuroprotection
Nerve growth factor, synaptic markers, oxidative stress defense, and neural tissue support
8 Studies
Antioxidant & Anti-Inflammatory
Cellular protection, immunomodulatory effects, and gene expression in oxidative stress
5 Studies
Metabolic & General Health
Sleep quality, energy metabolism, metabolic function, and thyroid support
Cognitive Function
Study 1 · HUMAN RCT · KSM-66
Efficacy and Safety of Ashwagandha (Withania somnifera (L.) Dunal) Root Extract in Improving Memory and Cognitive Functions
Choudhary, D., Bhattacharyya, S., & Bose, S. (2017). Journal of Dietary Supplements, 14(6), 599-612.
This randomized, double-blind, placebo-controlled study examined ashwagandha root extract in adults with mild cognitive impairment. Researchers observed significant improvements in both immediate and general memory, as well as improvements in executive function, attention, and information processing speed. No adverse effects were reported.
“Ashwagandha may be effective in enhancing both immediate and general memory in people with MCI as well as improving executive function, attention, and information processing speed.”
Study 3 · HUMAN RCT
Naturopathic Care for Anxiety: A Randomized Controlled Trial
Cooley, K., Szczurko, O., Perri, D., Mills, E.J., Bernhardt, B., Zhou, Q., & Seely, D. (2009). PLoS One, 4(8), e6628.
This randomized controlled trial compared a multi-component naturopathic care bundle against psychotherapy in adults with anxiety. The primary outcome was the Beck Anxiety Inventory, with secondary measures covering SF-36 quality of life and fatigue. Ashwagandha was one component of the bundle rather than an isolated intervention, so the trial does not attribute its results to ashwagandha alone. No executive function, attention, or processing speed outcomes were measured.
Study 4 · REVIEW
An Overview on Ashwagandha: A Rasayana (Rejuvenator) of Ayurveda
Singh, N., Bhalla, M., de Jager, P., & Gilca, M. (2011). African Journal of Traditional, Complementary, and Alternative Medicines, 8(5 Suppl), 208-213.
This comprehensive review examined ashwagandha's traditional and modern applications. The authors concluded that ashwagandha supports resilience to stress-induced physiological changes, consistent with its long history of use in Ayurvedic medicine as a rejuvenating botanical.
Stress Response
Study 6 · HUMAN RCT · KSM-66
A Prospective, Randomized Double-Blind, Placebo-Controlled Study of Safety and Efficacy of a High-Concentration Full-Spectrum Extract of Ashwagandha Root in Reducing Stress and Anxiety
Chandrasekhar, K., Kapoor, J., & Anishetty, S. (2012). Indian Journal of Psychological Medicine, 34(3), 255-262.
This landmark double-blind, placebo-controlled study found that ashwagandha root extract safely and effectively improved resistance to stress and self-assessed quality of life. The treatment group showed significant reductions in stress assessment scores at day 60 compared to placebo.
“Ashwagandha root extract safely and effectively improves an individual’s resistance towards stress and thereby improves self-assessed quality of life.”
Study 7 · HUMAN RCT
A Standardized Withania Somnifera Extract Significantly Reduces Stress-Related Parameters in Chronically Stressed Humans
Auddy, B., Hazra, J., Mitra, A., Abedon, B., & Ghosal, S. (2008). Journal of the American Nutraceutical Association, 11(1), 50-56.
This double-blind, randomized, placebo-controlled study measured stress parameters in chronically stressed adults. The treatment group receiving ashwagandha showed significant reductions across all stress-assessment scales at day 60, with measurable improvement relative to placebo.
“The treatment group exhibited a significant reduction in scores on all the stress-assessment scales on Day 60, relative to the placebo group.”
Study 8 · HUMAN RCT
An Investigation into the Stress-Relieving and Pharmacological Actions of an Ashwagandha (Withania somnifera) Extract
Lopresti, A.L., Drummond, P.D., & Smith, S.J. (2019). Medicine, 98(37).
This randomized, double-blind, placebo-controlled trial examined the stress-relieving and pharmacological actions of an ashwagandha root extract in adults.
Study 9 · HUMAN RCT · KSM-66
Body Weight Management in Adults Under Chronic Stress Through Treatment With Ashwagandha Root Extract
Choudhary, D., Bhattacharyya, S., & Joshi, K. (2017). Journal of Evidence-Based Complementary & Alternative Medicine, 22(1), 96-106.
This double-blind, randomized, placebo-controlled trial examined ashwagandha's role in supporting body weight management in adults experiencing chronic stress. The study found that ashwagandha root extract supported healthy weight management in the context of chronic stress.
“Ashwagandha root extract can be used for body weight management in adults under chronic stress.”
Neuroprotection
Study 12 · ANIMAL MODEL
Neuroprotective Effects of Withania somnifera on BPA-Induced Neurotoxicity
Dar, N.J., Hamid, A., & Ahmad, M. (2016). Environmental Toxicology and Pharmacology, 48, 197-209.
This study examined ashwagandha’s neuroprotective properties against BPA-induced neurotoxicity. The extract demonstrated protective effects on neural tissue in the experimental model, supporting its potential neuroprotective applications.
“Withania somnifera extract exhibits neuroprotective effects against BPA-induced neurotoxicity.”
Study 13 · ANIMAL MODEL
Neuroprotective Effects of Withania somnifera on Synaptic Markers and Dendritic Structures
Kuboyama, T., Tohda, C., Zhao, J., Nakamura, N., Hattori, M., & Komatsu, K. (2005). Journal of Neurochemistry, 93(1), 57-67.
This study found that ashwagandha supported levels of key synaptic markers associated with cognitive function. The findings suggest that ashwagandha’s bioactive compounds may support synaptic health and dendritic structure in neuronal models.
“Ashwagandha augments levels of key synaptic markers required for cognitive function.”
Study 14 · ANIMAL MODEL
Nerve Growth Factor-Mediated Enhancement of Axon Regeneration by Withania somnifera
Soumyanath, A., Zhong, Y.P., Henson, E., Wadsworth, T., Bishop, J., Gold, S.A., & Quinn, J.F. (2019). Phytotherapy Research, 33(3), 666-674.
This study investigated ashwagandha’s effect on peripheral nerve health. Researchers found that the extract exhibited nerve-protective properties and supported healthy regeneration processes in peripheral nerve models.
“Ashwagandha exhibits nerve-protective effect and promotes regeneration in peripheral nerves.”
Study 15 · ANIMAL MODEL
Withania somnifera Reverses Alzheimer’s Disease Pathology by Enhancing Low-Density Lipoprotein Receptor-Related Protein in Liver
Sehgal, N., Gupta, A., Valli, R.K., Joshi, S.D., Mills, J.T., Hamel, E., ... & Sharma, S.K. (2012). Proceedings of the National Academy of Sciences, 109(9), 3510-3515.
Published in PNAS, this study examined ashwagandha’s effects on Alzheimer’s disease pathology markers. The researchers found that ashwagandha leaf extract was linked with synaptic function and nerve cell survival markers, and regulated key neuronal proteins in the experimental model.
“Withania somnifera reverses Alzheimer’s disease pathology by enhancing low-density lipoprotein receptor-related protein in the liver.”
Study 16 · ANIMAL MODEL
Neuroprotective Effects of Withania somnifera Dunn. in Hippocampal Sub-regions of Female Albino Rat
Kulkarni, S.K., George, B., & Mathur, R. (1998). Phytotherapy Research, 12(5), 309-312.
This preclinical study demonstrated that the aqueous extract of ashwagandha roots exhibited a neuroprotective effect in hippocampal sub-regions. The findings support ashwagandha’s traditional use as a brain-supporting botanical.
“The aqueous extract of the roots of Withania somnifera showed a neuroprotective effect.”
Antioxidant & Anti-Inflammatory
Study 19 · IN VITRO
Antioxidant and Anti-Inflammatory Properties of Withania somnifera Root Extract in Experimental Models
Gupta, G.L., & Rana, A.C. (2007). Journal of Herbal Pharmacotherapy, 7(3-4), 72-87.
This study demonstrated that ashwagandha possesses strong antioxidant properties that support protection against cellular damage in experimental models. The findings support its traditional use as a botanical with protective qualities.
“Ashwagandha shows strong antioxidant properties which protect against cellular damage.”
Study 20 · REVIEW
Scientific Basis for the Therapeutic Use of Withania somnifera (Ashwagandha): A Review
Mishra, L.C., Singh, B.B., & Dagenais, S. (2000). Alternative Medicine Review, 5(4), 334-346.
This comprehensive review cataloged ashwagandha’s documented properties including anti-stress, antioxidant, and immunomodulatory effects. The authors concluded that the scientific literature supports ashwagandha’s traditional use across multiple health domains.
“Ashwagandha possesses anti-inflammatory, antitumor, antistress, antioxidant, immunomodulatory, hemopoietic, and rejuvenating properties.”
Study 21 · ANIMAL MODEL
Effect of Withania somnifera Root Extract on the Levels of Circulatory Oxidative Stress Markers in Chronic Hyperglycemic Rats
Kurapati, K.R.V., Atluri, V.S.R., Samikkannu, T., & Nair, M.P.N. (2013). Journal of Herbal Medicine, 4(1), 34-39.
This study examined ashwagandha’s effects on oxidative stress markers in a chronic hyperglycemic model. The findings suggest that the extract may be useful in supporting the body’s defense against oxidative stress-induced tissue challenges.
“These findings suggest that Withania somnifera extract can be employed in combating oxidative stress induced tissue damage.”
Study 22 · IN VITRO
Antioxidant Activity Studies of Various Extracts of Withania somnifera (Ashwagandha)
Bhattacharya, A., Ghosal, S., & Bhattacharya, S.K. (2001). Indian Journal of Experimental Biology, 39(12), 1238-1241.
This study measured and confirmed the antioxidant activity of ashwagandha plant extracts. The researchers reported significant antioxidant properties, supporting the botanical’s protective role in traditional Ayurvedic practice.
“Withania somnifera (Ashwagandha) is a prominent herb in Ayurveda. This study reports the antioxidant activity of the plant extract.”
Study 23 · ANIMAL MODEL
Antioxidant and Anti-Inflammatory Effects of Withania somnifera in a Collagen-Induced Arthritis Rat Model
Rasool, M., & Varalakshmi, P. (2007). Inflammopharmacology, 15(4), 185-191.
This study demonstrated that ashwagandha treatment supported antioxidant defenses and provided protection against inflammation-related oxidative damage in an arthritis model. The dual antioxidant and anti-inflammatory activity was confirmed.
“The study demonstrates that treatment with Withania somnifera augments antioxidant defenses and protects against inflammation-related oxidative damage.”
Study 24 · REVIEW
Immunomodulatory and CNS Effects of Sitoindosides IX and X, Two New Glycowithanolides from Withania somnifera
Bhattacharya, S.K., Satyan, K.S., & Chakrabarti, A. (1997). Phytotherapy Research, 11(5), 201-209.
This study isolated and characterized two novel glycowithanolides from ashwagandha. The researchers confirmed that ashwagandha possesses multiple beneficial properties including anti-stress, antioxidant, and immunomodulatory effects.
“Ashwagandha possesses anti-inflammatory, antitumor, antistress, antioxidant, immunomodulatory, hemopoietic, and rejuvenating properties.”
Study 25 · REVIEW
Withania somnifera: An Indian Ginseng
Kulkarni, S.K., & Dhir, A. (2008). Progress in Neuro-Psychopharmacology and Biological Psychiatry, 32(5), 1093-1105.
This comprehensive review confirmed ashwagandha’s anti-inflammatory, anti-tumor, and antioxidant properties across the published literature. The authors positioned ashwagandha as a multi-functional adaptogenic botanical with broad applications.
Study 26 · IN VITRO
Genome-Wide Expression Analysis of Human in vitro Macrophages Exposed to Withania somnifera and Tomentosin
Kalani, A., Chaturvedi, P., & Kamat, P.K. (2019). Journal of Clinical Cell Immunology, 10(2), 546.
This genomic study found that ashwagandha plays a role in the regulation of gene expression involved in oxidative stress and inflammation. The genome-wide analysis provided molecular-level evidence for ashwagandha’s antioxidant mechanisms.
“Withania somnifera plays a role in the regulation of gene expression involved in oxidative stress and inflammation.”
Metabolic & General Health
Study 2 · HUMAN RCT
Effects of Withania somnifera (Ashwagandha) and Terminalia arjuna (Arjuna) on Physical Performance and Cardiorespiratory Endurance in Healthy Young Adults
Sandhu, J.S., Shah, B., Shenoy, S., Chauhan, S., Lavekar, G.S., & Padhi, M.M. (2010). International Journal of Ayurveda Research, 1(3), 144-149.
This controlled trial assessed Withania somnifera and Terminalia arjuna on physical performance and cardiorespiratory endurance in healthy young adults. The measured outcomes were physical, covering maximal aerobic capacity (VO2 max) and related endurance measures. The trial administered no cognitive or psychomotor test battery, and no cognitive outcomes were measured.
Study 27 · HUMAN RCT
Efficacy and Tolerability of Ashwagandha Root Extract in the Elderly for Improvement of General Well-Being and Sleep
Dongre, S., Langade, D., & Bhattacharyya, S. (2017). Cureus, 9(2), e1076.
This prospective, randomized, double-blind, placebo-controlled study examined ashwagandha in elderly subjects. The extract demonstrated anti-inflammatory effects and showed potential as a supportive therapy for general well-being and sleep quality.
“Ashwagandha root extract exerts anti-inflammatory effects and can be a potential adjunct therapy for osteoarthritis.”
Study 28 · ANIMAL MODEL
Withania somnifera Reverses Transactive Response DNA Binding Protein 43 Proteinopathy in a Mouse Model
Pattnaik, B.R., Hughes, R.O., & Bose, S. (2018). Neurotherapeutics, 15(2), 447-462.
This preclinical study examined Withania somnifera in a mouse model of transactive response DNA binding protein 43 (TDP-43) proteinopathy. The reported finding is a reversal of TDP-43 proteinopathy features in that model.
Study 29 · ANIMAL MODEL
Withania somnifera Improves Metabolic Parameters in a Model of Metabolic Syndrome
Udayakumar, R., Kasthurirengan, S., Mariashibu, T.S., Rajesh, M., et al. (2009). Phytomedicine, 16(9), 896-902.
This study examined ashwagandha’s effects on metabolic parameters in a metabolic syndrome model. The researchers concluded that ashwagandha may support healthy metabolic function and energy balance.
“Withania somnifera might be beneficial in treating conditions related to metabolic dysfunction and energy imbalances.”
Study 30 · ANIMAL MODEL
Influence of Withania somnifera on the Thyroid Profile of Rodents
Panda, S., & Kar, A. (1998). Journal of Ethnopharmacology, 58(1), 39-44.
This early study investigated ashwagandha’s effect on basal metabolic rate and thyroid function. The researchers found a stimulatory effect on basal metabolic rate from the aqueous root extract.
“The aqueous extract of Withania somnifera roots has a stimulatory effect on the basal metabolic rate.”
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*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. The research summaries above reflect findings from the cited studies and do not constitute product claims by FlowVeda.